Highly sensitive green spectrofluorimetric method for determination of metoclopramide via enhancement of its native fluorescence by micellar formation with sodium dodecyl sulfate

Document Type : Research Articles

Authors

1 Analytical Chemistry Department, Faculty of Pharmacy, Port Said University

2 Al azhar University Faculty of Pharmacy Assiut Branch

Abstract

A new simple, accurate, green, a highly sensitive, non-extractable, economic, and rapid spectrofluorimetric method was developed for the determination of metoclopramide hydrochloride (MTC) in its pharmaceutical formulations and spiked human plasma. The method was based on enhancement of the native fluorescence of the studied drug via micellar formation with an anionic surfactant sodium dodecyl sulfate (SDS). The formed micellar fluorescence was measured at 347 nm after excitation at 310 nm. The micellar formation with SDS leads to enhancement of native fluorescence for MTC by about 550%. The fluorescence intensity was directly proportional to the drug concentrations over the concentration range of 25-1500 ng ml-1. The detection limit of 8 ng ml-1 and with a quantitation limit of 24 ng ml-1 .The proposed analytical method was validated according to ICH guidelines and the results were acceptable. The proposed analytical method has been successfully applied for the determination of the studied drugs in its pharmaceutical formulations as well as spiked human plasma and gives an excellent % recovery. The results show excellent agreement with the reported method with respect to precision and accuracy. The greenness of the proposed method was assessed by GAC principle and AGREE method.

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